Closed GFP Cam
Chlorophyll Fluorescence Imaging from Individual Cells to Plant Populations
About...

Closed GFPCam FC 800-C/1010GFP was specifically developed for various applications requiring imaging of green fluorescent proteins in molecular and cellular biology. The device is equipped with a motorized, software-controlled filter wheel, which provides quick and simple switching between the GFP and chlorophyll fluorescence detection.

Key Features

  • Combines imaging of GFP and chlorophyll fluorescence
  • Dark room for adaptation
  • Four super bright LED panels available in numerous wavelengths
  • Adjustable shelf system for different plant sizes


  • High-resolution CCD camera
  • Supplied with a laptop computer and software
  • Additional top stand mounted LED panel (optional)

Closed GFP Cam


Closed GFPCam FC 800-C/1010GFP is a GFP version of the Closed FluorCam and represents a highly innovative, robust and user-friendly world-wide used system for combined multispectral and kinetic fluorescence imaging. The basic system of the Closed FluorCam (CCD camera, four fixed LED panels) is supplemented by a fully motorized and software-controlled filter wheel and appropriate filter sets for the detection and imaging of green fluorescent protein (GFP), red-shifted GFP (EGFP), or other variants of fluorescent proteins. The blue emitting and red fluorescent protein can be detected and imaged in absence of chlorophyll. It consists of a CCD camera, four to five fixed LED panels (4 + 1 additional, which is not included in the standard setup) and of a filter wheel equipped with up to 7 different emission filters. The LED panels provide uniform irradiance over an area 90 × 90 mm –suitable for imaging of small plants (such as Arabidopsis thaliana), detached leaves, mosses, lichens, plated algal colonies or algal suspensions, etc. The system is very compact and allows easy dark adaptation of an investigated sample.

The Closed GFPCam FluorCam generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance is determined by user-defined protocols. The device configuration also provides user-friendly and quick switching between the GFP and chlorophyll fluorescence detection.

The Closed GFPCam FluorCam includes a high-performance PC and comprehensive software package comprising full system control, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.



Protocols


  • FV/FM
  • Kautsky induction
  • Quenching analysis
  • Light Curve
  • Steady state fluorescence, e.g.,ChlF, GFP and other FPs
  • Multicolor fluorescence, e.g., UV induced plant blue-green and chlorophyll fluorescence (F440,F520, F680, F740)
  • QA-reoxidation (it needs optional electronic module)
  • Fast fluorescence induction (OJIP) with 1 µs resolution (it needs optional electronic module)
  • PAR absorptivity (it needs additional far-red LED panel)



Samples


  • Leaves, small plants, fruits, vegetables
  • Mosses, lichens
  • Cyanobacteria, green algae
  • Nematodes
  • Selectable shelf system for different plant size
  • LED panels 130 x 130 mm for uniform illumination over sample area
  • Predefined imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
  • Dark room for adaptation



Applications


  • GFP detection and expression studies
  • Multicolor fluorescence
  • Screening for photosynthetic performance and metabolic perturbations
  • Detection of biotic and abiotic stress
  • Plant’s resistance or susceptibility to various stress factors
  • Plant molecular biology, screening for mutants
  • Plant-microbe interactions



Software


  • Fully automated control of the whole FluorCam system
  • Image acquisition via automated experimental protocols:
  • - numerous predefned protocols
    - possibility to create user defned protocols via a program Wizard
    - multiple (automatically repeated) experiments
    - barcode reader support

  • Image processing tools:
  • - automatic or manual image segmentation – e.g., labeling of individual plants
    - analysis of kinetic data from all samples within the feld of view
    - numerous image manipulation tools
    - export to text fle, avi, bmp or raw data formats

  • Windows 7, or higher compatible



For more information about LED wavelengths options and CCD cameras go to Products Introduction


Specifications


Fluorescence Parameters:
- Measured parameters: F0, FM, FV, F0', FM', FV', FT
- More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII, NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, and many others

Light Sources:
455 nm, 470 nm, 505 nm, 570 nm, 605 nm, 618 nm, 630 nm, 735 nm, white and others

Super Pulse Intensity:
- 4,000 µmol. m-2. s-1 (in standard version)
- 6,000 µmol. m-2. s-1 (in light-upgraded version)

Actinic Light Intensity:
Up to 2,000 µmol. m-2. s-1 (in standard version)
Up to 3,000 µmol. m-2. s-1 (in light-upgraded version)

Filter Wheel:
7 positions

Light Regime:
Static or harmonically modulated (sine waveform)

Custom-Defined Protocols:
Variable timing, special language and scripts

CCD Detector Wavelength Range:
400 – 1000 nm

CCD Format:
- 720 x 560 pixels (TOMI-1 camera)
- 1360 x 1024 pixels (TOMI-2 camera)
- 1280 x 1024 pixels (TOMI-3 camera)

Pixel Size:
- 8.20 µm x 8.40 µm (TOMI-1)
- 6.45 µm x 6.45 µm (TOMI-2)
- 6.60 µm x 6.60 µm (TOMI-3)

A/D bit Resolution:
- 12 bit (TOMI-1)
- 16 bit (TOMI-2)
- 12 bit (TOMI-3)

Interface Connector:
Gigabit Ethernet

Outer Dimension:
472 mm (W) x 479 mm (D) x 513 mm (H)

Weight:
Appr. 40 kg

Power Input:
Appr. 1100 W

Electrical:
90 – 240 V

Camera:
High-resolution TOMI-2

For more information about LED wavelengths options and CCD cameras go to Products Introduction



Optional features and accessories


QA-Reoxidation Module
High-end option allowing determination of QA-reoxidation kinetics. QA-reoxidation is recorded in a pump-and-probe manner, i.e. using repetitive flashes and recording with variable delay after each flash.

PAR-Absorptivity and NDVI Module
High-end option for measuring PAR absorptivity and NDVI reflectance index. It includes 7-position filter wheel, light panel 13 x 13 cm (installed round the camera and mounted with far-red LEDs 740 nm + deep-red LEDs 660 nm) and a glass filter. PAR-measurement is based on reflectance of plants in near infrared region and in the visible red range of spectrum. NDVI is used as vegetative index for predicting photosynthetic activity.
IMPORTANT NOTES: To be used only with FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010. This module cannot be used with the Multicolor Plant Module in FC 800-C/1010 or FC 800-C/1010-GPF.

Multicolor Plant Module
High-end option for measuring emission spectra in plants (according to Lichtenthaler and Bushmann). It includes 7-position filter wheel, UV LED light panel (installed round the camera and mounted with 4 UV LEDs 380 nm), four filters - BLUE (FF01-440/40), GREEN (FF02-520/28), RED (FF01-690/8), FAR-RED (FF01-747/33) - and a special software module.
IMPORTANT NOTES: To be used only in the FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010. This module cannot be used with the PAR-Absorptivity and NDVI Module in the FC 800-C/1010 or FC 800-C/1010-GPF.

Fast Fluorescence Induction (OJIP) Module
Electronic module for tracking fast fluorescence induction – OJIP curve. Includes a special electronic board and measuring protocol. The processes are recorded in multiple light exposures, each with a variable delay after the light is switched on.

Filter Wheel
7-position software-controlled filter wheel allows for imaging in different emission bands. Filters are easily interchangeable.

Additional LED Light Panels
Additional LED light panel is mounted at the top part of the FluorCam System. It is located under the camera; the objective of the camera is inserted in the central opening of this panel. The panel widens the possibility of employing more excitation colors for special applications. It is equipped with 4 high-power LEDs in one-channel version, or 8 high-power LEDs in two-channel version. Available colours are: far-red (740 nm), deep-red (660 nm), ultra-violet (365 or 385 nm), green (523 nm), amber (590 nm). Other options available upon request.
Ultraviolet Panel (365 or 385 nm): Recommended use: (1) multicolor fluorescence - UV excited blue F440, green F520, red F680, and infra-red F740 (must be accompanied with the filter wheel and high-resolution CCD); (2) excitation source for wtGFP, DAPI (filter wheel and high-resolution CCD required). Optical power 1,5 W.
Blue Panel (470 nm): Recommended use: (1) excitation source for EGFP (must be accompanied with the filter wheel and high-resolution CCD); (2) studies on stomata function.
Green Panel (530 nm): Recommended use: excitation source for YFP (must be accompanied with the filter wheel and high-resolution CCD).
Far-Red Panel (740 nm) : Maximum total output: 1,200 mW (4 x 300 mW). Recommended use: determination of Fo' and PAR absorbance (use of filter wheel required).
PAR - NDVI Panel (660 nm + 740 nm): This panel is part of the PAR-Absorptivity and NDVI Module.

Set of Grids for Sample Holders
Grids serve for firm and secure attachment of sample holders - Petri dishes or multi-well plates - to the desired spot on the FluorCam shelf or bottom. They are sold as a set of six different holders: for 90 mm, 100 mm, 120 mm, or 140 mm Petri dishes; 4 x 60 mm Petri dish; and for 96-well plate.

Additional Protective Glasses
Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.

Advanced Multiple Function for FluorCam Software
Software upgrade enabling multiple scripting functions for automatic initiation and measurement of scheduled FluorCam protocols and for automatic data storage. Advanced multiple function feature allows to define time of protocol initiation with user-defined protocol, apply user defined mask for feature segmentation, automatically subtract the background based on the mask definition and analyze the measured data. The are stored as TAR files with time and date description. Useful for circadian cycle study.

Comprehensive Module for Automatic and Programmable Image Acquisition and Data Management *
Comprehensive software and hardware package for data acquisition and image parameters extraction and analysis. Software part offers user friendly graphical interface, where complex protocols can be easily designed including dark/light adaptation schemes and executed based on user defined actions. All acquired data are stored in an SQL database, processed and available for inspection and further analysis in range of seconds after recording. System allows rapid and easy data browsing, grouping, analysis, user-defined data reprocessing and export. Hardware part includes PC with RAID5 data array, router, monitor, keyboard and mouse.

Barcode Reader with Supporting Software
Barcode reader with FluorCam supporting software included. Used for fully automatic bar code search.

Pricing


FluorCam
Version Characteristics Price
Closed GFPCam FC 800-C/1010-GFP Includes: FluorCam control unit, high-resolution camera, motorized filter wheeel with filters for GFP and Chl fluorescence, 4 light-upgraded LED panels 130 x 130 mm (red-orange and blue), control PC, software package, user’s guide. 24,850 €

Optional Accessories

Additional Far-Red Light Panel Recommended use for determination of Fo' and PAR absorbance. Must be accompanied with filter wheel. 1,990 €
Additional Ultraviolet Light Panel Recommended use: multicolor fluorescence. Should be accompanied with the filter wheel and high-resolution camera. 2,990 €
Additional Single Color Light Panel Recommended use: excitation source for EGFP, YFP, etc. Should be accompanied with the filter wheel and high-resolution camera. 2,490 €
Additional Bi-Color Light Panel Should be accompanied with the filter wheel and high-resolution camera. 2,790 €
QA-Reoxidation Module Allows performing of rapid measurements of QA-reoxidation kinetics (in Closed FluorCam only). 11,000 €
PAR Absorptivity and NDVI Module High-end option for measuring PAR absorptivity and NDVI reflectance index. It includes 7-position filter wheel, light panel 130 x 130 mm (installed round the camera and mounted with far-red LEDs 735 nm + red LEDs 650 nm) and a glass filter. To be used only with FC 800-C/1010, FC 800-C/1010-GFP or FC 800-O/1010. 5,480 €
Multicolor Plant Module * High-end option for measuring emission spectra in plants (according to Lichtenthaler and Bushmann). It includes 7-position filter wheel, UV LED light panel (installed round the camera and mounted with 4 UV LEDs 380 nm), four filters, and a special software module. 8,990 €
Fast Fluorescence Induction (OJIP) Module Electronic module for tracking fast fluorescence induction – OJIP curve. Includes a special electronic board and measuring protocol. 6,900 €
Advanced Multiple Function for FluorCam Software Software upgrade enabling automatic multiple experiments (without an operator) in the FluorCam device. Useful for circadian cycle study. 400 €
Comprehensive Module for Automatic and Programmable Image Acquisition and Data Management * Comprehensive software and hardware package for data acquisition and image parameters extraction and analysis. 5,990 €
Set of Grids for Sample Holders Grids serve for firm and secure attachment of sample holders - Petri dishes or multi-well plates - to the desired spot on the FluorCam shelf or bottom. They are sold as a set of six different holders: for 90 mm, 100 mm, 120 mm, or 140 mm Petri dishes; 4 x 60 mm Petri dish; and for 96-well plate. 90 €
Barcode Reader with Supporting Software Barcode reader with FluorCam supporting software included. Used for fully automatic bar code search. 910 €
Additional Protective Glasses * Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge. 19 €