Open FluorCam
Chlorophyll Fluorescence Imaging from Individual Cells to Plant Populations
About...

Open FluorCam FC 800-O/1010 and FC 800-O/2020 are a highly modular instruments with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. Also, the position of the camera relative to the sample is adjustable.

Key Features

  • Four super bright LED panels available in specific wavelengths
  • Adjustable system for different plant sizes
  • Imaged area for camera
  • - TOMI-1: 180 ×140 mm
    - TOMI-2: 220 ×160 mm


  • Supplied with a notebook and software
  • High-resolution or high-sensitivity CCD camera
  • Dark room for adaptation (optional)
  • Additional top stand mounted LED panel (optional)
  • Additional exchangeable LED panels (optional)

Open FluorCam


Open FluorCam FC 800-O/1010 and FC 800-O/2020 are a highly modular instruments with flexible geometry enabling work with samples of various sizes (from microtiter plates to small trees). Open FluorCam FC 800-O represents a highly innovative, robust and user-friendly world-wide used system for combined multispectral and kinetic fluorescence imaging. It consists of a CCD camera, four to five fixed LED panels (4 + 1 additional, which is not included in the standard setup) and, optionally, of a filter wheel equipped with up to 7 different emission filters. The four LED panels can be arranged at various angles and distances from the sample. The position of the camera may also be adjusted with respect to the sample height.

The dimensions of the LED panels can be 130 × 130 mm or 200 × 200 mm, which is suitable for imaging of small plants (such as Arabidopsis thaliana) up to middle size plants or small tress, detached leaves, fruits, vegetables, etc. The system allows easy dark adaptation of an investigated sample if dark box (it is not part of the standard set-up).

The Open FluorCam FC 800-O/1010 generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The Open FluorCam FC 800-O also includes a high-performance PC and comprehensive software package comprising full system control, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.




Light Panels


  • Standard version of the FluorCam system includes four super bright LED panels - size 130 x 130 mm or 200 x 200 mm. One pair of LED panels provides Measuring Light and Actinic Light 1 (red-orange 617 nm - in standard version). Other two panels provide Actinic Light 2 and Saturating Pulse (cool white 6500 K - in standard version).
  • Other available wavelengths for light sources are: royal-blue (447 nm), blue (470 nm), green (530 nm), cyan (505 nm), red (627 nm), deep-red (655 nm), amber (590 nm) - these lights sources are mounted only in customized FluorCam versions.
  • Additional LED panel may be added to increase the colour spectrum of the light sources for multi-colour fluorescence measurements. Additional panel is mounted around the camera. Available colours are: far-red (740 nm), deep-red (660 nm), ultra-violet (365 or 385 nm), green (523 nm), amber (590 nm). Other options available upon request.
  • Actinic light intensity:
  • - 300-2,000 µmol(photons)/m².s in the standard version depending on light wavelength,
    - up to 3,000 µmol(photons)/m².s in the light-upgraded version - please inquire at: info@psi.cz
  • Super pulse intensity:
  • - up to 4,000 µmol(photons)/m².s (in the standard version),
    - up to 6,000 µmol(photons)/m².s (in the light-upgraded version - please inquire at: info@psi.cz
  • Single Turnover Flash (STF): 120,000 µmol(photons)/m².s in 100µs pulse (in the QA re-oxidation version)



Protocols


  • FV/FM
  • Kautsky induction
  • Quenching analysis
  • Light Curve
  • Steady state fluorescence, e.g., ChlF, GFP and other FPs (flter wheel required)
  • Multicolor fluorescence, e.g., UV induced plant blue-green fluorescence (F440, F520) and chlorophyll fluorescence (F680, F740); flter wheel required
  • PAR absorptivity (it needs optional accessories: flter wheel and additional far-red LED panel)



Measured Parameters


  • Measured parameters: FO, FM, FV, FO', FM', FV', FT
  • More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, ETR (via Light Curve protocol), PAR-absorptivity coefficient, and many others





Application


  • Screening for photosynthetic performance and metabolic perturbations
  • Detection of biotic and abiotic stress
  • Plant’s resistance or susceptibility to various stress factors
  • Yield Improvement
  • Agriculture and horticulture
  • Growth and development





Software


  • Fully automated control of the whole FluorCam system
  • Image acquisition via automated experimental protocols:
  • - numerous predefned protocols
    - possibility to create user defned protocols via a program Wizard
    - multiple (automatically repeated) experiments
    - barcode reader support

  • Image processing tools:
  • - automatic or manual image segmentation – e.g., labeling of individual plants
    - analysis of kinetic data from all samples within the feld of view
    - numerous image manipulation tools
    - export to text fle, avi, bmp or raw data formats

  • Windows 7, or higher compatible



For more information about LED wavelengths options and CCD cameras go to Products Introduction


Versions



Open FluorCam FC 800-O/1010-S

The system is intended for imaging of chlorophyll fluorescence. The standard setup includes: high-sensitivity camera 720 x 560 px, 4 LED-based panels 130 x 130 mm (imaged area smaller) generating measuring light, actinic light and saturating pulses (two panels with red-orange LEDs, two panels with white LEDs ), filter for Chl fluorescence, control PC with software package, power supply, stand holding the camera and panels, connecting cables, user's guide.


Open FluorCam FC 800-O/1010-CUST

The standard setup includes: High-sensitivity camera 720 x 560 px or high-resolution camera, 4 LED-based panels 130 x 130 mm (imaged area smaller) generating measuring light, actinic light and saturating pulses (selectable wavelengths), filter for Chl fluorescence (optionally other filter and/or filter wheel), control PC with software package, power supply, stand holding the camera and panels, connecting cables, user's guide.


Open GFP-Cam FC 800-O/1010-GFP

The system is intended for imaging of both chlorophyll fluorescence and green fluorescence protein (GFP). The system includes: high-resolution camera, 4 upgraded LED-based panels 130 x 130 mm (imaged area smaller) generating measuring light, actinic light and saturating pulses (two panels with red-orange LEDs, two panels with blue LEDs), 7-position motorized filter wheel with filters for Chl and GFP fluorescence, control PC with software package, power supply, stand holding the camera and panels, connecting cables, user's guide. It is highly recommended that the system is supplemented with a dark-adaptation box (optional item).


Open FluorCam FC 800-O/2020-S

The system is intended for imaging of chlorophyll fluorescence. The standard setup includes: High-sensitivity camera 720 x 560 px,, control unit, 4 LED-based panels 200 x 200 mm (imaged area smaller) generating measuring light, actinic light and saturating pulses (two panels with red-orange LEDs, two panels with white LEDs ), filter for Chl fluorescence, modular stand, high-performance PC, software package, connecting cables, user’s guide.


Open FluorCam FC 800-O/2020-CUST

The standard setup includes: High-sensitivity camera 720 x 560 px or high-resolution camera, 4 LED-based panels 200 x 200 mm (imaged area smaller) generating measuring light, actinic light and saturating pulses (selectable wavelengths), filter for Chl fluorescence (optionally other filters and/or filter wheel), control PC with software package, power supply, stand holding the camera and panels, connecting cables, user's guide.


Open FluorCam FC 800-O/2020-GFP

The system is intended for imaging of both chlorophyll fluorescence and green fluorescence protein (GFP). The system includes: high-resolution camera, 4 upgraded 4 LED-based panels 200 x 200 mm (imaged area smaller) generating measuring light, actinic light and saturating pulses (two panels with red-orange LEDs, two panels with blue LEDs ), 7-position motorized filter wheel with filters for Chl and GFP fluorescence, control PC with software package, power supply, stand holding the camera and panels, connecting cables, user's guide. It is highly recommended that the system is supplemented with a dark-adaptation box (optional item).

Specifications


Fluorescence Parameters:
- Measured parameters: F0, FM, FV, FO', FM', FV', FT
- More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII, NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, and many others

Light Sources:
455 nm, 470 nm, 505 nm, 570 nm, 605 nm, 618 nm, 630 nm, 735 nm, white and others

Super Pulse Intensity:
- 4,000 µmol. m-2. s-1 (in standard version)
- 6,000 µmol. m-2. s-1 (in light-upgraded version)

Actinic Light Intensity:
Up to 2,000 µmol. m-2. s-1 (in standard version)
Up to 3,000 µmol. m-2. s-1 (in light-upgraded version)

Filter Wheel:
7 positions

Light Regime:
Static or harmonically modulated (sine waveform)

Custom-Defined Protocols:
Variable timing, special language and scripts

Dimensions (W × D × H):
737 × 737 × 435 mm

Weight:
Appr. 40 kg

Power Input:
Appr. 1100 W

Electrical:
90 – 240 V

Cameras:
High-sensitivity TOMI-1 or high-resolution TOMI-2

For more information about LED wavelengths options and CCD cameras go to Products Introduction

Optional features and accessories


High Resolution Camera TOMI-2
This camera (resolution 1360 x 1024 px, 20 images per second) is intended for combined measurement of ChlF and detection of weak steady-state fluorescence signals (such as GFP) with long integration times or applications where the higher spatial resolution is of highest importance (microscopy).

PAR-Absorptivity and NDVI Module
High-end option for measuring PAR absorptivity and NDVI reflectance index. It includes 7-position filter wheel, light panel 13 x 13 cm (installed round the camera and mounted with far-red LEDs 740 nm + deep-red LEDs 660 nm) and a glass filter. PAR-measurement is based on reflectance of plants in near infrared region and in the visible red range of spectrum. NDVI is used as vegetative index for predicting photosynthetic activity. To be used only with FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010.

Multicolor Plant Module
High-end option for measuring emission spectra in plants (according to Lichtenthaler and Bushmann). It includes 7-position filter wheel, UV LED light panel (installed round the camera and mounted with 4 UV LEDs 380 nm), four filters - BLUE (FF01-440/40), GREEN (FF02-520/28), RED (FF01-690/8), FAR-RED (FF01-747/33) - and a special software module.
IMPORTANT NOTES: To be used only in the FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010. This module cannot be used with the PAR-Absorptivity and NDVI Module in the FC 800-C/1010 or FC 800-C/1010-GPF.

Filter Wheel
7-position software-controlled filter wheel allows for imaging in different emission bands. Filters are easily interchangeable.

Additional LED Light Panels
Additional LED light panel is mounted at the top part of the FluorCam System. It is located under the camera; the objective of the camera is inserted in the central opening of this panel. The panel widens the possibility of employing more excitation colors for special applications. It is equipped with 4 high-power LEDs in one-channel version, or 8 high-power LEDs in two-channel version. Available colours are: far-red (740 nm), deep-red (660 nm), ultra-violet (365 or 385 nm), green (523 nm), amber (590 nm). Other options available upon request.
Ultraviolet Panel (365 or 385 nm): Recommended use: (1) multicolor fluorescence - UV excited blue F440, green F520, red F680, and infra-red F740 (must be accompanied with the filter wheel and high-resolution CCD); (2) excitation source for wtGFP, DAPI (filter wheel and high-resolution CCD required). Optical power 1,5 W.
Blue Panel (470 nm): Recommended use: (1) excitation source for EGFP (must be accompanied with the filter wheel and high-resolution CCD); (2) studies on stomata function.
Green Panel (530 nm): Recommended use: excitation source for YFP (must be accompanied with the filter wheel and high-resolution CCD).
Far-Red Panel (740 nm) : Maximum total output: 1,200 mW (4 x 300 mW). Recommended use: determination of Fo' and PAR absorbance (use of filter wheel required).
PAR - NDVI Panel (660 nm + 740 nm): This panel is part of the PAR-Absorptivity and NDVI Module.

Set of Grids for Sample Holders
Grids serve for firm and secure attachment of sample holders - Petri dishes or multi-well plates - to the desired spot on the FluorCam shelf or bottom. They are sold as a set of six different holders: for 90 mm, 100 mm, 120 mm, or 140 mm Petri dishes; 4 x 60 mm Petri dish; and for 96-well plate.

Additional Protective Glasses
Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.

Dark Adaptation Box
Allows dark adaptation of measured samples and/or their adjustment to varying light conditions. Manufactured in two versions: Version 1010 is suitable for FluorCam FC 800-O/1010; Version 2020 is suitable for FluorCam FC 800-O/2020. (Due to the fact that both boxes are bulky and heavy, they are available for EU customers only.)

Advanced Multiple Function for FluorCam Software
Software upgrade enabling multiple scripting functions for automatic initiation and measurement of scheduled FluorCam protocols and for automatic data storage. Advanced multiple function feature allows to define time of protocol initiation with user-defined protocol, apply user defined mask for feature segmentation, automatically subtract the background based on the mask definition and analyze the measured data. The are stored as TAR files with time and date description. Useful for circadian cycle study.

Comprehensive Module for Automatic and Programmable Image Acquisition and Data Management *
Comprehensive software and hardware package for data acquisition and image parameters extraction and analysis. Software part offers user friendly graphical interface, where complex protocols can be easily designed including dark/light adaptation schemes and executed based on user defined actions. All acquired data are stored in an SQL database, processed and available for inspection and further analysis in range of seconds after recording. System allows rapid and easy data browsing, grouping, analysis, user-defined data reprocessing and export. Hardware part includes PC with RAID5 data array, router, monitor, keyboard and mouse.

Barcode Reader with Supporting Software
Barcode reader with FluorCam supporting software included. Used for fully automatic bar code search.

Pricing


FluorCam
Version Characteristics Price
Open FluorCam FC 800-O/1010-S The standard setup includes: High-sensitivity camera, 4 LED light panels 13 x 13 cm (red-orange + white), filters for Chl fluorescence, control PC with software package, power supply, stand holding the camera and panels, connecting cables, user's guide. 15,990 €
Open FluorCam FC 800-O/1010-CUST The standard setup includes: High-sensitivity camera, 4 LED light panels 13 x 13 cm (selectable wavelengths), filters for Chl fluorescence, control PC with software package, power supply, stand holding the camera and panels, connecting cables, user's guide. Please inquire
Open GFP-Cam FC 800-O/1010-GFP The system includes: high-resolution camera, 4 upgraded LED light panels 13 x 13 cm (two red-orange, two blue), 7-position motorized filter wheel with filters for Chl and GFP fluorescence, control PC with software package, power supply, stand holding the camera and panels, connecting cables, user's guide. 24,350 €
Open FluorCam FC 800-O/2020-S Includes a CCD camera, 4 LED panels 20 x 20 cm (red-orange + white), filter for Chl fluorescence, modular stand, control unit, high-performance personal computer, software package, user’s guide. Please inquire
Open FluorCam FC 800-O/2020-CUST Includes a CCD camera, 4 LED panels 20 x 20 cm (selectable wavelengths), filter for Chl fluorescence, modular stand, control unit, high-performance personal computer, software package, user’s guide. Please inquire
Open GFP-Cam FC 800-O/2020-GFP The system includes: high-resolution camera, 4 upgraded LED light panels 20 x 20 cm (red-orange and blue), 7-position motorized filter wheel with filters for Chl and GFP fluorescence, control PC with software package, power supply, stand holding the camera and panels, connecting cables, user's guide. Please inquire

Optional Accessories

Additional Far-Red Light Panel Recommended use for determination of Fo' and PAR absorbance. Must be accompanied with filter wheel. 1,990 €
Additional Ultraviolet Light Panel Recommended use: multicolor fluorescence. Should be accompanied with the filter wheel and high-resolution camera. 2,990 €
Additional Single Color Light Panel Recommended use: excitation source for EGFP, YFP, etc. Should be accompanied with the filter wheel and high-resolution camera. 2,490 €
Additional Bi-Color Light Panel Should be accompanied with the filter wheel and high-resolution camera. 2,790 €
Surcharge for High Resolution Camera TOMI-2 * Camera with resolution 1360 x 1024 px. Intended for combined measurement of ChlF and detection of weak steady-state fluorescence signals (such as GFP). 4,000 €
7-Position Filter Wheel Motorized filter wheel allows for imaging in different emission bands (software controlled). 3,490 €
QA-Reoxidation Module Allows performing of rapid measurements of QA-reoxidation kinetics (in Closed FluorCam only). 11,000 €
PAR Absorptivity and NDVI Module High-end option for measuring PAR absorptivity and NDVI reflectance index. It includes 7-position filter wheel, light panel 13 x 13 cm (installed round the camera and mounted with far-red LEDs 735 nm + red LEDs 650 nm) and a glass filter. To be used only with FC 800-C/1010, FC 800-C/1010-GFP or FC 800-O/1010. 5,480 €
Multicolor Plant Module * High-end option for measuring emission spectra in plants (according to Lichtenthaler and Bushmann). It includes 7-position filter wheel, UV LED light panel (installed round the camera and mounted with 4 UV LEDs 380 nm), four filters, and a special software module. 8,990 €
Fast Fluorescence Induction (OJIP) Module Electronic module for tracking fast fluorescence induction – OJIP curve. Includes a special electronic board and measuring protocol. 6,900 €
Advanced Multiple Function for FluorCam Software Software upgrade enabling automatic multiple experiments (without an operator) in the FluorCam device. Useful for circadian cycle study. 400 €
Comprehensive Module for Automatic and Programmable Image Acquisition and Data Management * Comprehensive software and hardware package for data acquisition and image parameters extraction and analysis. 5,990 €
Set of Grids for Sample Holders Grids serve for firm and secure attachment of sample holders - Petri dishes or multi-well plates - to the desired spot on the FluorCam shelf or bottom. They are sold as a set of six different holders: for 90 mm, 100 mm, 120 mm, or 140 mm Petri dishes; 4 x 60 mm Petri dish; and for 96-well plate. 90 €
Barcode Reader with Supporting Software Barcode reader with FluorCam supporting software included. Used for fully automatic bar code search. 910 €
Additional Protective Glasses * Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge. 19 €