Handy FluorCam
Chlorophyll Fluorescence Imaging from Individual Cells to Plant Populations
About...

Handy FluorCam FC 1000-H is a lightweight, portable device designed for time resolved chlorophyll fluorescence imaging of leaves, small plants, leaf segments, mosses, lichens, seeds, roots, tissues on plates or algal colonies both in a field and laboratory.

Key Features

  • Both lab and field applications
  • Portable system (151 ×151 × 263 mm, 280 mm height with leaf clip)


  • AC & battery powered
  • Supplied with a PC and software
  • High sensitivity or High-resolution CCD camera

Handy FluorCam


Handy FluorCam FC 1000-H is a lightweight, portable device designed for time resolved chlorophyll fluorescence imaging of leaves, small plants, leaf segments, mosses, lichens, seeds, roots, tissues on plates or algal colonies both in a field and laboratory. The typical size of an investigated object is up to 41.5 × 31.5 mm. Handy FluorCam is a compact instrument consisting of a CCD camera and four fixed LED panels, one pair supplying the measuring pulses and the second pair providing actinic illumination and saturating flash. Handling of larger leaves (e.g., tobacco) is simplified if an additional leaf clip with gentle lock/release mechanism is mounted. The Handy FluorCam FC 1000-H is an easily portable system, it can be powered from the power supply or it can run on batteries provided in
a convenient bag carried on the shoulder.

Handy FluorCam FC 1000-H generates images of fluorescence signal at any moment of the experiment and presents them using
a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The Handy FluorCam is delivered with a laptop computer preinstalled with a comprehensive software package comprising full system control, Wizard with the most frequently used experimental protocols, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.


Light Sources


  • Four super bright LED light panels 40 x 40 mm
  • Imaged area 35 x 46 mm
  • Excitation light colors:
    • - measuring pulses: red-orange (620 nm)
    - actinic / saturating light: white – standard version, blue (455 nm), red-orange (620 nm)
    - far-red 735 nm

  • Saturating pulse intensity:
    • - white: up to 3,900 µmol.m-2. s-1
    - blue: up to 4,900 µmol.m-2. s-1
    - red-orange: up to 3,800 µmol.m-2. s-1

  • Actinic light intensity:
    • - white: approx. 1,000 µmol.m-2. s-1
    - blue: approx. 1,400 µmol.m-2. s-1
    - red-orange: approx. 800 µmol.m-2. s-1
  • Light regime:
    • - Static or sinus form (hub connected)


Protocols


  • FV/FM
  • Kautsky induction
  • Quenching analysis
  • Light Curve


Measured Parameters


  • Measured parameters: FO, FM, FV, FO', FM', FV', FT
  • More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, ETR (via Light Curve protocol), PAR-absorptivity coefficient, and many others



Samples


  • Leaves, small plants, fruits, vegetables
  • Mosses, lichens
  • Cyanobacteria, green algae
  • Nematodes
  • Predefined imaging masks for 384-well plate, 96-well plate, Petri dish, etc.



Applications


  • Screening for photosynthetic performance and metabolite perturbations
  • Detection of biotic and abiotic stress
  • Plant’s resistance or susceptibility to various stress factors
  • Plant-microbe interactions
  • Agriculture and horticulture
  • Yield improvement


Software


  • Fully automated control of the whole FluorCam system
  • Image acquisition via automated experimental protocols:
    • - numerous predefined protocols
    - possibility to create user defined protocols via a program Wizard
    - multiple (automatically repeated) experiments
    - barcode reader support

  • Image processing tools:
    • - automatic or manual image segmentation – e.g., labeling of individual plants
    - analysis of kinetic data from all samples within the field of view
    - numerous image manipulation tools
    - export to text file, avi, bmp or raw data formats

  • Windows 7, or higher compatible


For more information about LED wavelengths options and CCD cameras go to Products Introduction

Specifications


Fluorescence Parameters:
- Measured parameters: F0, FM, FV, F0', FM', FV', FT
- More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII, NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, and many others

Excitation Light Sources:
Measuring pulses:red-orange (620 nm);
Actinic/saturating light:white (standard),red-orange (620 nm) or blue (455 nm)

Saturating Pulse Illumination:
White: Up to 3,900 µmol.m-2. s-1
Blue: Up to 4,900 µmol.m-2. s-1
Red-orange: Up to 3,800 µmol.m-2. s-1

Actinic Illumination:
White: 1,000 µmol.m-2. s-1
Blue: Up to 1,400 µmol.m-2. s-1
Red-orange: Up to 800 µmol.m-2. s-1

Light Regime:
Static or sine waveform (hub connected)

Custom-Defined Protocols:
Variable timing, special language and scripts

Handy FluorCam Weight:
1.8 kg

Leaf clip Weight:
0.2 kg

Power Supply Weight:
2.5 kg

FluorCam Stand Weight:
1.5 kg

Notebook Weight (incl. all accessories):
3.5 kg

Power Input:
Max. 200 W

Electrical:
90 – 260 V

Dimensions
(W × D × H): 151 × 151 × 263 mm, 280 mm height with leaf clip

Cameras:
High-sensitivity TOMI-1 or High-resolution TOMI-2

For more information about LED wavelengths options and CCD cameras go to Products Introduction


Optional features and accessories


High Resolution Camera TOMI-2
This camera (resolution 1360 x 1024 px, 20 images per second) is intended for combined measurement of ChlF and detection of weak steady-state fluorescence signals (such as GFP) with long integration times or applications where the higher spatial resolution is of highest importance (microscopy).

Ultra-Light Tripod
The tripod stand is a very useful accessory for field experiments. Its robust and flexible construction offers needed stability during imaging operations. The stand is water resistant and its variable height allows imaging of samples at different levels within the vegetation. Height up to 1.5 m; weight 4 kg; includes a bag.

Leaf Clip
The leaf clip simplifies the process of collecting fluorescence images on vegetation. The unique design includes a gentle lock/release system for holding the target leaf in place without detachment or excessive disturbance. It also allows for dark adaptation.

Battery Pack
A useful supplement for comfortable field measurements. Weight: 3.6 kg; size: 220 x 200 x 130 mm.

Additional Protective Glasses
Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.

Advanced Multiple Function for FluorCam Software
Software upgrade enabling multiple scripting functions for automatic initiation and measurement of scheduled FluorCam protocols and for automatic data storage. Advanced multiple function feature allows to define time of protocol initiation with user-defined protocol, apply user defined mask for feature segmentation, automatically subtract the background based on the mask definition and analyze the measured data. The are stored as TAR files with time and date description. Useful for circadian cycle study.

[h1]Documents[/h1] [list] [*][b] FluorCam Operation Manual (pdf)[/b] [*][b] FluorCam Quick Start Guide (pdf)[/b] [*][b] Driver Instalation manual (pdf)[/b] [/list] [h1]Software[/h1] [list] [*] [b] FluorCam 7 (installer), OS: Win 7/10[/b] [*] [b] Legacy driver for USB cameras, OS: Win 7 (32 bit)[/b] [/list] [h1]Promotional Materials[/h1] [list] [*][b] FluorCams leaflet (pdf)[/b] [/list] [h1]Tutorial video[/h1] [list] [*][b] FluorCam Tutorial video (mp4)[/b] [/list]

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