Handy GFP Cam
Chlorophyll Fluorescence Imaging from Individual Cells to Plant Populations
About...

Handy GFPCam FC 1000-H/GFP is a modified version of the popular Handy FluorCam. Its unique construction allows both time resolved chlorophyll fluorescence imaging and GFP imaging. Handy GFPCam is a lightweight, portable system designed for imaging of leaves, small plants, leaf segments, mosses, lichens, seeds, roots, tissues on plates or algal colonies both in a field and laboratory.

Key Features

  • Combined imaging of GFP and chlorophyll fluorescence
  • Both lab and field applications
  • Portable system 151 ×151 × 263 mm,
  • AC & battery powered


  • Supplied with a laptop computer and software
  • High-resolution CCD camera
  • Imaged area: 35 × 46 mm

Handy GFP Cam


Handy GFPCam FC 1000-H/GFP is a modified version of the popular Handy FluorCam. Its unique construction allows both time resolved chlorophyll fluorescence imaging and GFP imaging. Handy GFPCam is a lightweight, portable system designed for imaging of leaves, small plants, leaf segments, mosses, lichens, seeds, roots, tissues on plates or algal colonies both in a field and laboratory. The typical size of an investigated object is up to 35 × 46 mm.

The Handy GFPCam is an easily portable system, it can be powered from the power supply or it can run on batteries provided in a convenient bag carried on the shoulder.

Handy GFPCam is typically produced with red-orange flashing LED panels (620 nm) and blue actinic/saturating pulse LED panels (455 nm). Equipped with four additional infrared LEDs. Handling of larger leaves (e.g., tobacco) is simplified if an additional leaf clip with gentle lock/release mechanism is mounted. Handy GFPCam generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols.

The Handy GFPCam is delivered with a laptop computer preinstalled with a comprehensive software package comprising full system control, Wizard with the most frequently used experimental protocols, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.


Light Sources


  • Imaged area: 35 x 46 mm
  • Excitation light colors:
  • - measuring pulses: orange (620 nm), blue (455 nm)
    - actinic / saturating light: blue (455 nm)
    - far-red 735 nm

  • Saturating pulse intensity:
  • - blue: up to 6,000 µmol.m-2. s-1

  • Actinic light intensity:
  • - blue: approx. 1,500 µmol.m-2. s-1

  • Light regime:
  • - Static or sinus form (hub connected)



Protocols


  • Steady-state GFP fluorescence
  • FV/FM
  • Kautsky induction
  • Quenching analysis
  • Light Curve



Measured Parameters


  • Measured parameters: FO, FM, FV, FO', FM', FV', FT
  • More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, ETR (via Light Curve protocol), PAR-absorptivity coefficient, and many others




Applications


  • Screening for GFP-containing GMOs
  • Transgene expression and localization on whole-plant level
  • Screening for photosynthetic performance
  • Detection of biotic and abiotic stress
  • Plant’s resistance or susceptibility to various stress factors
  • Plant-microbe interactions





Software


  • Fully automated control of the whole FluorCam system
  • Image acquisition via automated experimental protocols:
  • - numerous predefined protocols
    - possibility to create user defined protocols via a program Wizard
    - multiple (automatically repeated) experiments
    - barcode reader support

  • Image processing tools:
  • - automatic or manual image segmentation – e.g., labeling of individual plants
    - analysis of kinetic data from all samples within the field of view
    - numerous image manipulation tools
    - export to text file, avi, bmp or raw data formats

  • Windows 7, or higher compatible



For more information about LED wavelengths options and CCD cameras go to Products Introduction

Specifications


Fluorescence Parameters:
- Measured parameters: F0, FM, FV, F0', FM', FV', FT
- More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII, NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, and many others

Excitation Light Sources:
Measuring pulses:red-orange (620 nm); blue (455 nm)
Actinic/saturating light: blue (455 nm),

Saturating Pulse Illumination:
Blue: Up to 6.000 µmol.m-2. s-1

Actinic Illumination:
Blue: Up to 1.500 µmol.m-2. s-1

Light Regime:
Static or sine waveform (hub connected)

Handy FluorCam Weight:
1.8 kg

Leaf clip Weight:
0.2 kg

Power Supply Weight:
2.5 kg

FluorCam Stand Weight:
1.5 kg

Notebook Weight (incl. all accessories):
3.5 kg

Power Input:
Max. 200 W

Electrical:
90 – 260 V

Dimensions
(W × D × H): 151 × 151 × 263 mm, 280 mm height with leaf clip

Camera:
High-resolution TOMI-2

For more information about LED wavelengths options and CCD cameras go to Products Introduction



Optional features and accessories


Ultra-Light Tripod
The tripod stand is a very useful accessory for field experiments. Its robust and flexible construction offers needed stability during imaging operations. The stand is water resistant and its variable height allows imaging of samples at different levels within the vegetation. Height up to 1.5 m; weight 3.3 kg; includes a bag.

Leaf Clip
The leaf clip simplifies the process of collecting fluorescence images on vegetation. The unique design includes a gentle lock/release system for holding the target leaf in place without detachment or excessive disturbance. It also allows for dark adaptation.

Battery Pack
A useful supplement for comfortable field measurements. Weight: 3.6 kg; size: 220 x 200 x 130 mm.

Additional Protective Glasses
Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.

Advanced Multiple Function for FluorCam Software
Software upgrade enabling multiple scripting functions for automatic initiation and measurement of scheduled FluorCam protocols and for automatic data storage. Advanced multiple function feature allows to define time of protocol initiation with user-defined protocol, apply user defined mask for feature segmentation, automatically subtract the background based on the mask definition and analyze the measured data. The are stored as TAR files with time and date description. Useful for circadian cycle study.

Pricing


FluorCam
Version Characteristics Price
Handy GFPCam FC 1000-H/GFP Includes high-sensitivity camera, 4 LED panels 40 x 40 mm (two red-orange, two blue), filter for Chl fluorescence, control unit, control PC, software package, user's guide. 18,990 €

Optional Accessories

Leaf Clip Useful supplement to the Handy FluorCam. Minimizes sample manipulation and allows dark adaptation. 390 €
Ultra-Light Tripod * Useful supplement to the Handy FluorCam in field experiments. Comes with a bag; weight 3.3 kg. 490 €
Battery Pack * Useful supplement to the Handy Version FluorCam for comfortable field experiments. Weight 3.6 kg. Bag size 220 x 200 x 130 mm. 490 €
Advanced Multiple Function for FluorCam Software Software upgrade enabling automatic multiple experiments (without an operator) in the FluorCam device. Useful for circadian cycle study. 400 €
Additional Protective Glasses * Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge. 19 €