Closed FluorCam FC 800-C is a popular, best-selling system for imaging of chlorophyll fluorescence kinetics which can be extended to multispectral version allowing detection of various fluorescent signals. The standard version consists of a CCD camera and four fxed LED panels. The LED panels provide uniform irradiance over samples up to 90 × 90 mm – suitable for small plants, detached leaves, algal dilutions, etc. The system allows dark adaptation.
The Closed FluorCam FC 800-C generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The Closed FluorCam FC 800-C also includes a high-performance PC and comprehensive software package comprising full system control, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.
Light Sources:
455 nm, 470 nm, 505 nm, 570 nm, 605 nm, 618 nm, 630 nm, 735 nm, white and others
Super Pulse Intensity:
- 4,000 µmol. m-2. s-1 (in standard version)
- 6,000 µmol. m-2. s-1 (in light-upgraded version)
Actinic Light Intensity:
Up to 2,000 µmol. m-2. s-1 (in standard version)
Up to 3,000 µmol. m-2. s-1 (in light-upgraded version)
Filter Wheel:
7 positions
Light Regime:
Static or harmonically modulated (sine waveform)
Custom-Defined Protocols:
Variable timing, special language and scripts
CCD Detector Wavelength Range:
400 – 1000 nm
CCD Format:
- 1360 x 1024 pixels (TOMI-2 camera)
- 1280 x 1024 pixels (TOMI-3 camera)
Pixel Size:
- 6.45 µm x 6.45 µm (TOMI-2)
- 6.60 µm x 6.60 µm (TOMI-3)
A/D bit Resolution:
- 16 bit (TOMI-2)
- 12 bit (TOMI-3)
Interface Connector:
Gigabit Ethernet
Outer Dimension:
472 mm (W) x 479 mm (D) x 513 mm (H)
Weight:
Appr. 40 kg
Power Input:
Appr. 1100 W
Electrical:
90 – 240 V
Cameras:
High-resolution TOMI-2 or highspeed TOMI-3
PAR-Absorptivity and NDVI Module
High-end option for measuring PAR absorptivity based on NDVI reflectance index. PAR absorptivity coefficient is calculated according to the equation a = 1- (red signal / near infra-red signal).
It includes 7-position filter wheel, light panel 130 x 130 mm (installed round the camera and mounted with far-red LEDs 735 nm + red LEDs 650 nm) and a glass filter. PAR-measurement is based on reflectance of plants in near infrared region and in the visible red range of spectrum. NDVI is used as vegetative index for predicting photosynthetic activity.
IMPORTANT NOTES: To be used only with FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010. This module cannot be used with the Multicolor Plant Module in FC 800-C/1010 or FC 800-C/1010-GPF.
Multicolor Plant Module
High-end option for measuring emission spectra in plants (according to Lichtenthaler and Bushmann). It includes 7-position filter wheel, UV LED light panel (installed round the camera and mounted with 4 UV LEDs 380 nm), four filters - BLUE (FF01-440/40), GREEN (FF02-520/28), RED (FF01-690/8), FAR-RED (FF01-747/33) - and a special software module.
IMPORTANT NOTES: To be used only in the FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010. This module cannot be used with the PAR-Absorptivity and NDVI Module in the FC 800-C/1010 or FC 800-C/1010-GPF.
Fast Fluorescence Induction (OJIP) Module
Electronic module for tracking fast fluorescence induction – OJIP curve. Includes a special electronic board and measuring protocol. The processes are recorded in multiple light exposures, each with a variable delay after the light is switched on.
Filter Wheel
7-position software-controlled filter wheel allows for imaging in different emission bands. Filters are easily interchangeable.
Additional LED Light Panels
Additional LED light panel is mounted at the top part of the FluorCam System. It is located under the camera; the objective of the camera is inserted in the central opening of this panel. The panel widens the possibility of employing more excitation colors for special applications. It is equipped with 4 high-power LEDs in one-channel version, or 8 high-power LEDs in two-channel version. Available colours are: far-red (740 nm), deep-red (660 nm), ultra-violet (365 or 385 nm), green (523 nm), amber (590 nm). Other options available upon request.
Ultraviolet Panel (365 or 385 nm): Recommended use: (1) multicolor fluorescence - UV excited blue F440, green F520, red F680, and infra-red F740 (must be accompanied with the filter wheel and high-resolution CCD); (2) excitation source for wtGFP, DAPI (filter wheel and high-resolution CCD required). Optical power 1,5 W.
Blue Panel (470 nm): Recommended use: (1) excitation source for EGFP (must be accompanied with the filter wheel and high-resolution CCD); (2) studies on stomata function.
Green Panel (530 nm): Recommended use: excitation source for YFP (must be accompanied with the filter wheel and high-resolution CCD).
Far-Red Panel (740 nm) : Maximum total output: 1,200 mW (4 x 300 mW). Recommended use: determination of Fo' and PAR absorbance (use of filter wheel required).
PAR - NDVI Panel (660 nm + 740 nm): This panel is part of the PAR-Absorptivity and NDVI Module.
Set of Grids for Sample Holders
Grids serve for firm and secure attachment of sample holders - Petri dishes or multi-well plates - to the desired spot on the FluorCam shelf or bottom. They are sold as a set of six different holders: for 90 mm, 100 mm, 120 mm, or 140 mm Petri dishes; 4 x 60 mm Petri dish; and for 96-well plate.
Additional Protective Glasses
Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.
Advanced Multiple Function for FluorCam Software
Software upgrade enabling multiple scripting functions for automatic initiation and measurement of scheduled FluorCam protocols and for automatic data storage. Advanced multiple function feature allows to define time of protocol initiation with user-defined protocol, apply user defined mask for feature segmentation, automatically subtract the background based on the mask definition and analyze the measured data. The are stored as TAR files with time and date description. Useful for circadian cycle study.
Comprehensive Module for Automatic and Programmable Image Acquisition and Data Management *
Comprehensive software and hardware package for data acquisition and image parameters extraction and analysis. Software part offers user friendly graphical interface, where complex protocols can be easily designed including dark/light adaptation schemes and executed based on user defined actions. All acquired data are stored in an SQL database, processed and available for inspection and further analysis in range of seconds after recording. System allows rapid and easy data browsing, grouping, analysis, user-defined data reprocessing and export. Hardware part includes PC with RAID5 data array, router, monitor, keyboard and mouse.
Barcode Reader with Supporting Software
Barcode reader with FluorCam supporting software included. Used for fully automatic bar code search.
Version | Characteristics | Price |
---|---|---|
Closed FluorCam FC 800-C/1010-S | Includes high-resolution camera, 4 LED panels 130 x 130 mm (two red-orange, two white), filter for Chl fluorescence, control unit, control PC, software package, user’s guide. | 19,290 € |
Closed FluorCam FC 800-C/1010-CUST | Includes high-resolution camera, 4 LED panels 130 x 130 mm (selectable wavelengths), filter for Chl fluorescence, control unit, control PC, software package, user’s guide. | Please inquire |
Optional Accessories
Additional Far-Red Light Panel |
Recommended use for determination of Fo'. | 2,149 € |
Additional Ultraviolet Light Panel |
Recommended use: multicolor fluorescence. Should be accompanied with the filter wheel and high-resolution camera. | 3,229 € |
Additional Single Color Light Panel |
Recommended use: excitation source for EGFP, YFP, etc. Should be accompanied with the filter wheel and high-resolution camera. | 2,690 € |
Additional Bi-Color Light Panel |
Should be accompanied with the filter wheel and high-resolution camera. | 2,999 € |
7-Position Filter Wheel | Motorized filter wheel allows for imaging in different emission bands (software controlled). | 3,770 € |
Thermoregulator TR6000 | Temperature module ensures precise and stable thermal control of live biological specimen. The module allows for either constant-temperature incubation or dynamic heating and cooling, following pre-set temperature profiles like rapid or linear heating with a uniform rate. The TR6000 comprises a Control Unit, a 48-well Thermal Block and Thermoregulator GUI control software which enables the synchronization of FluorCam experiments with TR6000. The TR GUI communication is exclusively supported in Fluorcam 10 software. | 11,880 € |
QA-Reoxidation Module | Allows performing of rapid measurements of QA-reoxidation kinetics (in Closed FluorCam only). | 11,880 € |
PAR Absorptivity and NDVI Module |
High-end option for measuring PAR absorptivity based on NDVI reflectance index. PAR absorptivity coefficient is calculated according to the equation a = (near infrared reflectance - red reflectance)/(near infrared reflectance + red reflectance) It includes 7-position filter wheel, light panel 130 x 130 mm (installed round the camera and mounted with far-red LEDs 735 nm + red LEDs 650 nm) and a glass filter. To be used only with FC 800-C/1010, FC 800-C/1010-GFP or FC 800-O/1010. |
5,920 € |
Multicolor Plant Module * | High-end option for measuring emission spectra in plants (according to Lichtenthaler and Bushmann). It includes 7-position filter wheel, UV LED light panel (installed round the camera and mounted with 4 UV LEDs 380 nm), four filters, and a special software module. | 9,690 € |
Fast Fluorescence Induction (OJIP) Module |
Electronic module for tracking fast fluorescence induction – OJIP curve. Includes a special electronic board and measuring protocol. | 7,455 € |
FluorCam 10 software (without a Laptop) | The latest generation of 64-bit control software for FluorCam devices with an array of enhanced tools aimed at data analysis, visualization, mass export of data. Additionally, it offers three optional extensions specifically designed to support various setups for benchtop-based automated measurements. The software is delivered on a USB flash drive. (Requirements: PC with Win10 or higher, a decent x64-based processor, 16 GB RAM, storage 1 TB, a Gigabit Ethernet network card.) | 2,700 € |
FluorCam 10 Software with a Pre-installed Laptop | The latest generation of 64-bit control software for FluorCam devices with an array of enhanced tools aimed at data analysis, visualization, mass export of data. Additionally, it offers three optional extensions specifically designed to support various setups for benchtop-based automated measurements. The software is delivered on a pre-installed laptop (64-bit operating system, x64-based processor, 16 GB of RAM, 256 GB SSD + 1 TB HDD storage, a Gigabit Ethernet network card essential for the connection with the FluorCam device). | 3,900 € |
Advanced Multiple Function for FluorCam 10 Software | Software upgrade enabling automated triggering of one measuring protocol. A straightforward wizard, utilizing pop-up windows, enables the input of the number of repetitions, the delay between measurements, and the adaptive lighting conditions before the measurement. The recorded data are saved with date & time stamp. The functionality is ideal for examining kinetic processes such as tracking pharmacological effects over time or exploration of circadian patterns. | 490 € |
Advanced Circadian Function for FluorCam 10 Software | The automatic function was specially designed for the study of circadian rhythms. It enables the repeated execution of two distinct measuring protocols: a day-time specific measurement and undisturbing monitoring of nocturnal changes. A straightforward wizard, utilizing pop-up windows, enables the input of the number of repetitions for up to two protocols, the delay between day and night measurements, and the adaptive lighting conditions for each phase of the day. The recorded data are saved with date & time stamp. | 990 € |
Advanced Multi-phase Function for FluorCam 10 Software | The automated extension feature enables the merging of n-phases, each with its unique protocol. This function unlocks the range of potential applications for semi-automated measurements. A simple wizard, utilizing pop-up windows, enables the input of the number of repetitions, the delay between measurements, and the adaptive lighting conditions before the measurement separately for each phase. The recorded data are saved with date & time stamp. | 1,690 € |
Comprehensive Module for Automatic and Programmable Image Acquisition and Data Management * |
Comprehensive software package empowers users with the ability to schedule experiments with extremely high degree of flexibility. It also enables automated data acquisition and automated analysis of data series stored in SQL database. Scheduling assistant with calendar function allows running multiple experiments simultaneously and sample lighting regime including dark/light adaptation. PlantScreenTM Data Analyser provides tools for data browsing, grouping, analysis, user-defined re-processing and export. | 6,490 € |
Set of Grids for Sample Holders |
Grids serve for firm and secure attachment of sample holders - Petri dishes or multi-well plates - to the desired spot on the FluorCam shelf or bottom. They are sold as a set of six different holders: for 90 mm, 100 mm, 120 mm, or 140 mm Petri dishes; 4 x 60 mm Petri dish; and for 96-well plate. | 99 € |
Extension for multi-well imaging |
Extension facilitates capability for inverted (upside-down) imaging of micro-plates. The set includes a specially designed shelf with a grid for fixed and defined placement of the microplates and supplementary legs providing extra support for ergonomic function of FC in an inverted configuration. | 65 € |
Barcode Reader with Supporting Software |
Barcode reader with FluorCam supporting software included. Used for fully automatic bar code search. | 990 € |
Additional Protective Glasses * | Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge. | 25 € |